Composite

Part:BBa_K2434006:Design

Designed by: Diana Priscilla Bonilla Ruelas   Group: iGEM17_TecMonterrey_GDA   (2017-09-24)


PHA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1108
    Illegal BglII site found at 5062
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 4351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4761
    Illegal BsaI.rc site found at 658
    Illegal BsaI.rc site found at 2875
    Illegal SapI.rc site found at 268


Design Notes

Optimized to work on E.coli


Source

This sequence has parts that have been taken from the iGEM parts registry, Pseudomonas aeruginosa, and Pseudomonas knackmussii b13.

References